other:
Article Title: The TCR-SUB1-DOCK2 axis promotes autoimmunity by driving pathogenic CD4 + T cell tissue infiltration.
Article Snippet: Article The TCR-SUB1-DOCK2 axis promotes autoimmunity by driving pathogenic CD4+ T cell tissue infiltration Highlights • SUB1 dictates the tissue infiltration of pathogenic CD4+ T cells • Sub1 expression is directly modulated by the TCR-IRF4 pathway • Sub1 deficiency prevents EAE onset by halving DOCK2 levels • SUB1 undergoes LLPS to open chromatin and drive the JUNB-DOCK2 transcriptional cascade Authors Xiaoxue Li, Wenhua Liang, Weifang Wang, Eilon Sherman, Keling Huang, Feng Wang Correspondence wangfeng16@sjtu.edu.cn In brief Blocking pathogenic T cell migration is crucial for treating autoimmune diseases.. Li et al. reveal SUB1 as a gatekeeper of CD4+ T cell infiltration in autoimmunity.. TCR-induced SUB1 drives Rac- dependent cytoskeletal remodeling via DOCK2 transcriptional activation.
Recombinant:Article Title: RORα is critical for mTORC1 activity in T cell-mediated colitis.
Article Snippet: .. Antibodies anti-CD3e eBioscience Cat# 45-0031-82; RRID: AB_1107000 anti-CD4 Biolegend Cat# 100548; RRID: AB_2563054 anti-CD69 BD Biosciences Cat# 557392; RRID: AB_396675 anti-CD127 eBioscience Cat# 48-1271-82; RRID: AB_2016698 anti-Gr1 eBioscience Cat# 48-5931-82; RRID: AB_1548788 anti-CD11b eBioscience Cat# 47-0112-82; RRID: AB_1603193 anti-Siglec F eBioscience Cat# 46-1702-80; RRID: AB_2573724 anti-a4b7 Biolegend Cat# 120608; RRID: AB_10730607 anti-CCR9 eBioscience Cat# 11-1991-82; RRID: AB_657513 anti-CD45 eBioscience Cat# 11-0451-82; RRID: AB_465050 anti-CD45.1 BD Biosciences Cat# 553775; RRID: AB_10926208 anti-CD27 Biolegend Cat# 124216; RRID: AB_10639726 anti-RORgt BD Biosciences Cat# 562607; RRID: AB_11153137 anti-FOXP3 eBioscience Cat# 48-5773-82; RRID: AB_1518812 anti-Bcl-2 Biolegend Cat# 633507; RRID: AB_2043939 anti-IL-17A eBioscience Cat# 48-7177-82; RRID: AB_11149503 anti-IFN-g BD Biosciences Cat# 557998; RRID: AB_396979 anti-TNFa BD Biosciences Cat# 17-7321-82; RRID: AB_469508 anti-mTOR pS2448 eBioscience Cat# 50-9718-42; RRID: AB_2574352 anti-4EBP1 pT36/pT45 BD Biosciences Cat# 560285; RRID: AB_1645498 anti-S6 pS235/pS236 BD Biosciences Cat# 25-9007-42; RRID: AB_2637099 anti-AKT pS473 BD Biosciences Cat# 560397; RRID: AB_1645553 anti-AKT pT308 BD Biosciences Cat# 558275; RRID: AB_2225329 anti-RORa ThermoFisher Cat# PA5-11224; RRID: AB_2285213 Donkey anti-rabbit IgG Biolegend Cat# 406406; RRID: AB_1575135 anti-CD4 (IF) Biolegend Cat# 100443; RRID: AB_2562557 anti-CD127 (IF) BD Biosciences Cat# 552543; RRID: AB_394417 anti-CD69 (IF) BD Biosciences Cat# 557392; RRID: AB_396675 anti-HA CST Cat# 3724 Normal Rabbit IgG CST Cat# 2729 Chemicals, peptides, and recombinant proteins Recombinant Human TGF-b R&D Systems Cat# 240-B-010 Recombinant Mouse IL-6 Peprotech Cat# 216-16 Recombinant Mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant Mouse IL-1b Peprotech Cat# 211-11B Critical commercial assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 FOXP3 / Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 ChIP-IT Express Enzymatic Shearing Kit NEXTflex Active Motif Cat# 53035 (Continued on next page) Cell Reports 36, 109682, September 14, 2021 e1 .. Deposited data RNA-seq, ChIP-seq and 16S-seq This study GSE168219 Effector/memory precursor CD8 (Kalia et al., 2010) GSE19825 Effector/stem-like Th17 (Karmaus et al., 2019) GSE107521 mTORC1 dependent genes in Th17 (Karmaus et al., 2019) GSE107521 Homeostatic/pathogenic Th17 (Omenetti et al., 2019) GSE130302 RORgt ChIP-seq (Ciofani et al., 2012) GSE40918 Th17 ATAC-seq (Chang et al., 2020) GSE153442 UC patients (Haberman et al., 2019) GSE109142 UC patients (Vancamelbeke et al., 2017) GSE75214 UC patients (Fenton et al., 2021) GSE128682 UC patients (Arijs et al., 2009) GSE14580 UC patients (Arijs et al., 2018) GSE73661 Experimental models: Organisms/strains Mouse: Rorafl/fl This study N/A Mouse: Cd4Cre (Lee et al., 2001) N/A Mouse: vav-Bcl2Tg (Ogilvy et al., 1999) N/A Mouse: C57BL/6J Jackson Laboratory JAX: 000664 Mouse: CD45.1 Jackson Laboratory JAX: 002014 Mouse: Tcrbd / Jackson Laboratory JAX: 002121 Mouse: Rptorfl/fl Dr. Bing Su lab N/A Software and algorithms FlowJo software v10.7 FlowJo LLC https://www.flowjo.com/ GraphPad Prism 7 GraphPad Software https://www.graphpad.com/ scientific-software/prism/ GSEA 4.0 N/A http://software.broadinstitute.org/gsea/
Article Title: Adipose-mesenchymal stromal cells suppress experimental Sjögren syndrome by IL-33-driven expansion of ST2 + regulatory T cells
Article Snippet: Statistical analysis was done with Graphpad Prism 7. .. Recombinant human IL-2 R&D Systems Cat# 202-IL-500 Recombinant human TGF-β1 R&D Systems Cat# 240-B-010 Recombinant mouse IL-1β PeproTech Cat# 211-11B Recombinant mouse IL-4 PeproTech Cat# 214-14 Recombinant mouse IL-5 PeproTech Cat# 215-15 Recombinant mouse IL-6 PeproTech Cat# 216-16 Recombinant mouse IL-7 PeproTech Cat# 217-17 Recombinant mouse IL-12 PeproTech Cat# 210-12 Recombinant mouse IL-13 PeproTech Cat# 210-13 Recombinant mouse IL-15 PeproTech Cat# 210-15 Recombinant mouse IL-21 R&D Systems Cat# 594-ML-025 Recombinant mouse IL-22 R&D Systems Cat# 582-ML-010 Recombinant mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant mouse/rat IL-33 R&D Systems Cat# M3300 Recombinant mouse TNF-α PeproTech Cat# 315-01A Recombinant mouse IFN-γ PeproTech Cat# 315-05 Recombinant Human IL-1β PeproTech Cat# 200-01B (5Z)-7-Oxozeaenol R&D Systems Cat# 3604/1 U0126 R&D Systems Cat# 1144/25 SB203580 R&D Systems Cat# 1202/10 SP600125 Invitrogen Cat# tlrl-sp60 JSH23 ab144824 abcam Cat#749886-87-1 PMA Sigma Aldrich Cat# P1585 Ionomycin Sigma Aldrich Cat# I0634 Golgi-Plug BD Biosciences Cat# 555029 TRIzol reagent Invitrogen Cat# 15596026 DNase I Sigma Alcrich Cat# DN25 Collagenase GIBCO Cat# 17104019 Regent or Resource source identifier RNeasy Mini Kit QIAGEN Cat# 74106 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368814 TaqMan Gene Expression Master Mix Applied Biosystems Cat# 4369016 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554714 CD4+ T cell isolation kit, mouse Miltenyi Biotec Cat # 130-104-456 CD4+CD25+ Regulatory T Cell Isolation Kit, mouse Miltenyi Biotec Cat# 130-091-041 .. hADSC Human Adipose-Derived Stem Cells Catalog #: PT-5006, Lonza Walkersville Inc
Article Title: Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Recombinant Mouse GM-CSF R&D Systems Cat#415-ML Recombinant Mouse IL-4 R&D Systems Cat#404-ML Recombinant Mouse IL-23 R&D Systems Cat#1887-ML-010 Recombinant Human GM-CSF R&D Systems Cat#215-GM Recombinant Human IL-4 R&D Systems Cat#204-IL Recombinant Murine Flt3-Ligand PeproTech Cat#250-31L L-arginine Sangon Biotech Cat#A600205 L-ornithine Sigma-Aldrich Cat#O6503 L-proline Sangon Biotech Cat#A600923 Creatine Sangon Biotech Cat#A600326 L-citrulline Sangon Biotech Cat#A604057 Spermidine Sigma-Aldrich Cat#S0266 nor-NOHA Cayman Cat#1140844-63-8 Oligomycin Abcam Cat#141829 DFMO Sigma-Aldrich Cat#D193 IMQ cream MedShine Cat#120503 Halometasone cream Bright Future Pharmaceutical Laboratories, Ltd. N/A Ribonuclease A Sigma-Aldrich Cat#R6513 HNRNPA1226-237 Genescript N/A TMR-labeled HNRNPA1226-237 Genescript N/A Krt1017-28 Genescript N/A CellTrace Violet Invitrogen Cat#C34557 ionomycin Sigma-Aldrich Cat#I9657 PMA Sigma-Aldrich Cat#79346 GolgiPlug BD Biosciences Cat#555029 Critical Commercial Assays Peroxidase Substrate Kit ZSGB-BIO Cat#ZLI-9018 Protein A/G magnetic beads Merck Millipore Cat#LSKMAGAG02 CD14 Microbeads Miltenyi Biotec Cat#130-050-201 TruSeq RNA sample preparation kit Illumina Cat#FC-122-1001 TruSeq paired-end cluster kit v3-cBot-HS Illumina Cat#PE-401-3001 M-MLV First-Strand Synthesis Kit Invitrogen Cat#C28025-021 Hieff qPCR SYBR Green Master Mix Yeasen Biotech Cat#11202ES03 Minute Endosome Isolation and Cell Fractionation Kit Invent Biotech Cat#ED-028 Mouse Cytokine Array GS4000 RayBiotech Cat#GSM-CAA-4000-1 Mouse IgE ELISA MAX Deluxe BioLegend Cat#432404 Mouse IL-6 ELISA kit NeoBioscience Cat#EMC004.96 Mouse TNF-a ELISA kit NeoBioscience Cat#EMC102a.96 Mouse IFN-alpha ELISA Kit R&D Systems Cat#42120-1 Human IL-6 ELISA Kit MultiSciences Cat#70-EK106/2-96 Human TNF-a High Sensitivity ELISA Kit MultiSciences Cat#70-EK182HS-96 SimpleCHIP enzymatic chromatin immunoprecipitation kit Cell Signaling Technology Cat#9002 Duolink In Situ Red Starter Kit Mouse/Rabbit Sigma-Aldrich Cat#DUO92101-1KT OxiSelect? .. Intracellular Nitric Oxide (NO) Assay Kit Cell Biolabs Cat#STA-800 ENLITEN ATP Assay System Promega Cat#FF2000 (Continued on next page) Immunity 53, 1–13.e1–e10, July 14, 2020 e2
Staining:Article Title: RORα is critical for mTORC1 activity in T cell-mediated colitis.
Article Snippet: .. Antibodies anti-CD3e eBioscience Cat# 45-0031-82; RRID: AB_1107000 anti-CD4 Biolegend Cat# 100548; RRID: AB_2563054 anti-CD69 BD Biosciences Cat# 557392; RRID: AB_396675 anti-CD127 eBioscience Cat# 48-1271-82; RRID: AB_2016698 anti-Gr1 eBioscience Cat# 48-5931-82; RRID: AB_1548788 anti-CD11b eBioscience Cat# 47-0112-82; RRID: AB_1603193 anti-Siglec F eBioscience Cat# 46-1702-80; RRID: AB_2573724 anti-a4b7 Biolegend Cat# 120608; RRID: AB_10730607 anti-CCR9 eBioscience Cat# 11-1991-82; RRID: AB_657513 anti-CD45 eBioscience Cat# 11-0451-82; RRID: AB_465050 anti-CD45.1 BD Biosciences Cat# 553775; RRID: AB_10926208 anti-CD27 Biolegend Cat# 124216; RRID: AB_10639726 anti-RORgt BD Biosciences Cat# 562607; RRID: AB_11153137 anti-FOXP3 eBioscience Cat# 48-5773-82; RRID: AB_1518812 anti-Bcl-2 Biolegend Cat# 633507; RRID: AB_2043939 anti-IL-17A eBioscience Cat# 48-7177-82; RRID: AB_11149503 anti-IFN-g BD Biosciences Cat# 557998; RRID: AB_396979 anti-TNFa BD Biosciences Cat# 17-7321-82; RRID: AB_469508 anti-mTOR pS2448 eBioscience Cat# 50-9718-42; RRID: AB_2574352 anti-4EBP1 pT36/pT45 BD Biosciences Cat# 560285; RRID: AB_1645498 anti-S6 pS235/pS236 BD Biosciences Cat# 25-9007-42; RRID: AB_2637099 anti-AKT pS473 BD Biosciences Cat# 560397; RRID: AB_1645553 anti-AKT pT308 BD Biosciences Cat# 558275; RRID: AB_2225329 anti-RORa ThermoFisher Cat# PA5-11224; RRID: AB_2285213 Donkey anti-rabbit IgG Biolegend Cat# 406406; RRID: AB_1575135 anti-CD4 (IF) Biolegend Cat# 100443; RRID: AB_2562557 anti-CD127 (IF) BD Biosciences Cat# 552543; RRID: AB_394417 anti-CD69 (IF) BD Biosciences Cat# 557392; RRID: AB_396675 anti-HA CST Cat# 3724 Normal Rabbit IgG CST Cat# 2729 Chemicals, peptides, and recombinant proteins Recombinant Human TGF-b R&D Systems Cat# 240-B-010 Recombinant Mouse IL-6 Peprotech Cat# 216-16 Recombinant Mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant Mouse IL-1b Peprotech Cat# 211-11B Critical commercial assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 FOXP3 / Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 ChIP-IT Express Enzymatic Shearing Kit NEXTflex Active Motif Cat# 53035 (Continued on next page) Cell Reports 36, 109682, September 14, 2021 e1 .. Deposited data RNA-seq, ChIP-seq and 16S-seq This study GSE168219 Effector/memory precursor CD8 (Kalia et al., 2010) GSE19825 Effector/stem-like Th17 (Karmaus et al., 2019) GSE107521 mTORC1 dependent genes in Th17 (Karmaus et al., 2019) GSE107521 Homeostatic/pathogenic Th17 (Omenetti et al., 2019) GSE130302 RORgt ChIP-seq (Ciofani et al., 2012) GSE40918 Th17 ATAC-seq (Chang et al., 2020) GSE153442 UC patients (Haberman et al., 2019) GSE109142 UC patients (Vancamelbeke et al., 2017) GSE75214 UC patients (Fenton et al., 2021) GSE128682 UC patients (Arijs et al., 2009) GSE14580 UC patients (Arijs et al., 2018) GSE73661 Experimental models: Organisms/strains Mouse: Rorafl/fl This study N/A Mouse: Cd4Cre (Lee et al., 2001) N/A Mouse: vav-Bcl2Tg (Ogilvy et al., 1999) N/A Mouse: C57BL/6J Jackson Laboratory JAX: 000664 Mouse: CD45.1 Jackson Laboratory JAX: 002014 Mouse: Tcrbd / Jackson Laboratory JAX: 002121 Mouse: Rptorfl/fl Dr. Bing Su lab N/A Software and algorithms FlowJo software v10.7 FlowJo LLC https://www.flowjo.com/ GraphPad Prism 7 GraphPad Software https://www.graphpad.com/ scientific-software/prism/ GSEA 4.0 N/A http://software.broadinstitute.org/gsea/
Article Title: Adipose-mesenchymal stromal cells suppress experimental Sjögren syndrome by IL-33-driven expansion of ST2 + regulatory T cells
Article Snippet: Statistical analysis was done with Graphpad Prism 7. .. Recombinant human IL-2 R&D Systems Cat# 202-IL-500 Recombinant human TGF-β1 R&D Systems Cat# 240-B-010 Recombinant mouse IL-1β PeproTech Cat# 211-11B Recombinant mouse IL-4 PeproTech Cat# 214-14 Recombinant mouse IL-5 PeproTech Cat# 215-15 Recombinant mouse IL-6 PeproTech Cat# 216-16 Recombinant mouse IL-7 PeproTech Cat# 217-17 Recombinant mouse IL-12 PeproTech Cat# 210-12 Recombinant mouse IL-13 PeproTech Cat# 210-13 Recombinant mouse IL-15 PeproTech Cat# 210-15 Recombinant mouse IL-21 R&D Systems Cat# 594-ML-025 Recombinant mouse IL-22 R&D Systems Cat# 582-ML-010 Recombinant mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant mouse/rat IL-33 R&D Systems Cat# M3300 Recombinant mouse TNF-α PeproTech Cat# 315-01A Recombinant mouse IFN-γ PeproTech Cat# 315-05 Recombinant Human IL-1β PeproTech Cat# 200-01B (5Z)-7-Oxozeaenol R&D Systems Cat# 3604/1 U0126 R&D Systems Cat# 1144/25 SB203580 R&D Systems Cat# 1202/10 SP600125 Invitrogen Cat# tlrl-sp60 JSH23 ab144824 abcam Cat#749886-87-1 PMA Sigma Aldrich Cat# P1585 Ionomycin Sigma Aldrich Cat# I0634 Golgi-Plug BD Biosciences Cat# 555029 TRIzol reagent Invitrogen Cat# 15596026 DNase I Sigma Alcrich Cat# DN25 Collagenase GIBCO Cat# 17104019 Regent or Resource source identifier RNeasy Mini Kit QIAGEN Cat# 74106 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368814 TaqMan Gene Expression Master Mix Applied Biosystems Cat# 4369016 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554714 CD4+ T cell isolation kit, mouse Miltenyi Biotec Cat # 130-104-456 CD4+CD25+ Regulatory T Cell Isolation Kit, mouse Miltenyi Biotec Cat# 130-091-041 .. hADSC Human Adipose-Derived Stem Cells Catalog #: PT-5006, Lonza Walkersville Inc
Chromatin Immunoprecipitation:Article Title: RORα is critical for mTORC1 activity in T cell-mediated colitis.
Article Snippet: .. Antibodies anti-CD3e eBioscience Cat# 45-0031-82; RRID: AB_1107000 anti-CD4 Biolegend Cat# 100548; RRID: AB_2563054 anti-CD69 BD Biosciences Cat# 557392; RRID: AB_396675 anti-CD127 eBioscience Cat# 48-1271-82; RRID: AB_2016698 anti-Gr1 eBioscience Cat# 48-5931-82; RRID: AB_1548788 anti-CD11b eBioscience Cat# 47-0112-82; RRID: AB_1603193 anti-Siglec F eBioscience Cat# 46-1702-80; RRID: AB_2573724 anti-a4b7 Biolegend Cat# 120608; RRID: AB_10730607 anti-CCR9 eBioscience Cat# 11-1991-82; RRID: AB_657513 anti-CD45 eBioscience Cat# 11-0451-82; RRID: AB_465050 anti-CD45.1 BD Biosciences Cat# 553775; RRID: AB_10926208 anti-CD27 Biolegend Cat# 124216; RRID: AB_10639726 anti-RORgt BD Biosciences Cat# 562607; RRID: AB_11153137 anti-FOXP3 eBioscience Cat# 48-5773-82; RRID: AB_1518812 anti-Bcl-2 Biolegend Cat# 633507; RRID: AB_2043939 anti-IL-17A eBioscience Cat# 48-7177-82; RRID: AB_11149503 anti-IFN-g BD Biosciences Cat# 557998; RRID: AB_396979 anti-TNFa BD Biosciences Cat# 17-7321-82; RRID: AB_469508 anti-mTOR pS2448 eBioscience Cat# 50-9718-42; RRID: AB_2574352 anti-4EBP1 pT36/pT45 BD Biosciences Cat# 560285; RRID: AB_1645498 anti-S6 pS235/pS236 BD Biosciences Cat# 25-9007-42; RRID: AB_2637099 anti-AKT pS473 BD Biosciences Cat# 560397; RRID: AB_1645553 anti-AKT pT308 BD Biosciences Cat# 558275; RRID: AB_2225329 anti-RORa ThermoFisher Cat# PA5-11224; RRID: AB_2285213 Donkey anti-rabbit IgG Biolegend Cat# 406406; RRID: AB_1575135 anti-CD4 (IF) Biolegend Cat# 100443; RRID: AB_2562557 anti-CD127 (IF) BD Biosciences Cat# 552543; RRID: AB_394417 anti-CD69 (IF) BD Biosciences Cat# 557392; RRID: AB_396675 anti-HA CST Cat# 3724 Normal Rabbit IgG CST Cat# 2729 Chemicals, peptides, and recombinant proteins Recombinant Human TGF-b R&D Systems Cat# 240-B-010 Recombinant Mouse IL-6 Peprotech Cat# 216-16 Recombinant Mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant Mouse IL-1b Peprotech Cat# 211-11B Critical commercial assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 FOXP3 / Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 ChIP-IT Express Enzymatic Shearing Kit NEXTflex Active Motif Cat# 53035 (Continued on next page) Cell Reports 36, 109682, September 14, 2021 e1 .. Deposited data RNA-seq, ChIP-seq and 16S-seq This study GSE168219 Effector/memory precursor CD8 (Kalia et al., 2010) GSE19825 Effector/stem-like Th17 (Karmaus et al., 2019) GSE107521 mTORC1 dependent genes in Th17 (Karmaus et al., 2019) GSE107521 Homeostatic/pathogenic Th17 (Omenetti et al., 2019) GSE130302 RORgt ChIP-seq (Ciofani et al., 2012) GSE40918 Th17 ATAC-seq (Chang et al., 2020) GSE153442 UC patients (Haberman et al., 2019) GSE109142 UC patients (Vancamelbeke et al., 2017) GSE75214 UC patients (Fenton et al., 2021) GSE128682 UC patients (Arijs et al., 2009) GSE14580 UC patients (Arijs et al., 2018) GSE73661 Experimental models: Organisms/strains Mouse: Rorafl/fl This study N/A Mouse: Cd4Cre (Lee et al., 2001) N/A Mouse: vav-Bcl2Tg (Ogilvy et al., 1999) N/A Mouse: C57BL/6J Jackson Laboratory JAX: 000664 Mouse: CD45.1 Jackson Laboratory JAX: 002014 Mouse: Tcrbd / Jackson Laboratory JAX: 002121 Mouse: Rptorfl/fl Dr. Bing Su lab N/A Software and algorithms FlowJo software v10.7 FlowJo LLC https://www.flowjo.com/ GraphPad Prism 7 GraphPad Software https://www.graphpad.com/ scientific-software/prism/ GSEA 4.0 N/A http://software.broadinstitute.org/gsea/
Article Title: Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Recombinant Mouse GM-CSF R&D Systems Cat#415-ML Recombinant Mouse IL-4 R&D Systems Cat#404-ML Recombinant Mouse IL-23 R&D Systems Cat#1887-ML-010 Recombinant Human GM-CSF R&D Systems Cat#215-GM Recombinant Human IL-4 R&D Systems Cat#204-IL Recombinant Murine Flt3-Ligand PeproTech Cat#250-31L L-arginine Sangon Biotech Cat#A600205 L-ornithine Sigma-Aldrich Cat#O6503 L-proline Sangon Biotech Cat#A600923 Creatine Sangon Biotech Cat#A600326 L-citrulline Sangon Biotech Cat#A604057 Spermidine Sigma-Aldrich Cat#S0266 nor-NOHA Cayman Cat#1140844-63-8 Oligomycin Abcam Cat#141829 DFMO Sigma-Aldrich Cat#D193 IMQ cream MedShine Cat#120503 Halometasone cream Bright Future Pharmaceutical Laboratories, Ltd. N/A Ribonuclease A Sigma-Aldrich Cat#R6513 HNRNPA1226-237 Genescript N/A TMR-labeled HNRNPA1226-237 Genescript N/A Krt1017-28 Genescript N/A CellTrace Violet Invitrogen Cat#C34557 ionomycin Sigma-Aldrich Cat#I9657 PMA Sigma-Aldrich Cat#79346 GolgiPlug BD Biosciences Cat#555029 Critical Commercial Assays Peroxidase Substrate Kit ZSGB-BIO Cat#ZLI-9018 Protein A/G magnetic beads Merck Millipore Cat#LSKMAGAG02 CD14 Microbeads Miltenyi Biotec Cat#130-050-201 TruSeq RNA sample preparation kit Illumina Cat#FC-122-1001 TruSeq paired-end cluster kit v3-cBot-HS Illumina Cat#PE-401-3001 M-MLV First-Strand Synthesis Kit Invitrogen Cat#C28025-021 Hieff qPCR SYBR Green Master Mix Yeasen Biotech Cat#11202ES03 Minute Endosome Isolation and Cell Fractionation Kit Invent Biotech Cat#ED-028 Mouse Cytokine Array GS4000 RayBiotech Cat#GSM-CAA-4000-1 Mouse IgE ELISA MAX Deluxe BioLegend Cat#432404 Mouse IL-6 ELISA kit NeoBioscience Cat#EMC004.96 Mouse TNF-a ELISA kit NeoBioscience Cat#EMC102a.96 Mouse IFN-alpha ELISA Kit R&D Systems Cat#42120-1 Human IL-6 ELISA Kit MultiSciences Cat#70-EK106/2-96 Human TNF-a High Sensitivity ELISA Kit MultiSciences Cat#70-EK182HS-96 SimpleCHIP enzymatic chromatin immunoprecipitation kit Cell Signaling Technology Cat#9002 Duolink In Situ Red Starter Kit Mouse/Rabbit Sigma-Aldrich Cat#DUO92101-1KT OxiSelect? .. Intracellular Nitric Oxide (NO) Assay Kit Cell Biolabs Cat#STA-800 ENLITEN ATP Assay System Promega Cat#FF2000 (Continued on next page) Immunity 53, 1–13.e1–e10, July 14, 2020 e2
Reverse Transcription:Article Title: Adipose-mesenchymal stromal cells suppress experimental Sjögren syndrome by IL-33-driven expansion of ST2 + regulatory T cells
Article Snippet: Statistical analysis was done with Graphpad Prism 7. .. Recombinant human IL-2 R&D Systems Cat# 202-IL-500 Recombinant human TGF-β1 R&D Systems Cat# 240-B-010 Recombinant mouse IL-1β PeproTech Cat# 211-11B Recombinant mouse IL-4 PeproTech Cat# 214-14 Recombinant mouse IL-5 PeproTech Cat# 215-15 Recombinant mouse IL-6 PeproTech Cat# 216-16 Recombinant mouse IL-7 PeproTech Cat# 217-17 Recombinant mouse IL-12 PeproTech Cat# 210-12 Recombinant mouse IL-13 PeproTech Cat# 210-13 Recombinant mouse IL-15 PeproTech Cat# 210-15 Recombinant mouse IL-21 R&D Systems Cat# 594-ML-025 Recombinant mouse IL-22 R&D Systems Cat# 582-ML-010 Recombinant mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant mouse/rat IL-33 R&D Systems Cat# M3300 Recombinant mouse TNF-α PeproTech Cat# 315-01A Recombinant mouse IFN-γ PeproTech Cat# 315-05 Recombinant Human IL-1β PeproTech Cat# 200-01B (5Z)-7-Oxozeaenol R&D Systems Cat# 3604/1 U0126 R&D Systems Cat# 1144/25 SB203580 R&D Systems Cat# 1202/10 SP600125 Invitrogen Cat# tlrl-sp60 JSH23 ab144824 abcam Cat#749886-87-1 PMA Sigma Aldrich Cat# P1585 Ionomycin Sigma Aldrich Cat# I0634 Golgi-Plug BD Biosciences Cat# 555029 TRIzol reagent Invitrogen Cat# 15596026 DNase I Sigma Alcrich Cat# DN25 Collagenase GIBCO Cat# 17104019 Regent or Resource source identifier RNeasy Mini Kit QIAGEN Cat# 74106 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368814 TaqMan Gene Expression Master Mix Applied Biosystems Cat# 4369016 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554714 CD4+ T cell isolation kit, mouse Miltenyi Biotec Cat # 130-104-456 CD4+CD25+ Regulatory T Cell Isolation Kit, mouse Miltenyi Biotec Cat# 130-091-041 .. hADSC Human Adipose-Derived Stem Cells Catalog #: PT-5006, Lonza Walkersville Inc
Gene Expression:Article Title: Adipose-mesenchymal stromal cells suppress experimental Sjögren syndrome by IL-33-driven expansion of ST2 + regulatory T cells
Article Snippet: Statistical analysis was done with Graphpad Prism 7. .. Recombinant human IL-2 R&D Systems Cat# 202-IL-500 Recombinant human TGF-β1 R&D Systems Cat# 240-B-010 Recombinant mouse IL-1β PeproTech Cat# 211-11B Recombinant mouse IL-4 PeproTech Cat# 214-14 Recombinant mouse IL-5 PeproTech Cat# 215-15 Recombinant mouse IL-6 PeproTech Cat# 216-16 Recombinant mouse IL-7 PeproTech Cat# 217-17 Recombinant mouse IL-12 PeproTech Cat# 210-12 Recombinant mouse IL-13 PeproTech Cat# 210-13 Recombinant mouse IL-15 PeproTech Cat# 210-15 Recombinant mouse IL-21 R&D Systems Cat# 594-ML-025 Recombinant mouse IL-22 R&D Systems Cat# 582-ML-010 Recombinant mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant mouse/rat IL-33 R&D Systems Cat# M3300 Recombinant mouse TNF-α PeproTech Cat# 315-01A Recombinant mouse IFN-γ PeproTech Cat# 315-05 Recombinant Human IL-1β PeproTech Cat# 200-01B (5Z)-7-Oxozeaenol R&D Systems Cat# 3604/1 U0126 R&D Systems Cat# 1144/25 SB203580 R&D Systems Cat# 1202/10 SP600125 Invitrogen Cat# tlrl-sp60 JSH23 ab144824 abcam Cat#749886-87-1 PMA Sigma Aldrich Cat# P1585 Ionomycin Sigma Aldrich Cat# I0634 Golgi-Plug BD Biosciences Cat# 555029 TRIzol reagent Invitrogen Cat# 15596026 DNase I Sigma Alcrich Cat# DN25 Collagenase GIBCO Cat# 17104019 Regent or Resource source identifier RNeasy Mini Kit QIAGEN Cat# 74106 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368814 TaqMan Gene Expression Master Mix Applied Biosystems Cat# 4369016 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554714 CD4+ T cell isolation kit, mouse Miltenyi Biotec Cat # 130-104-456 CD4+CD25+ Regulatory T Cell Isolation Kit, mouse Miltenyi Biotec Cat# 130-091-041 .. hADSC Human Adipose-Derived Stem Cells Catalog #: PT-5006, Lonza Walkersville Inc
Cell Isolation:Article Title: Adipose-mesenchymal stromal cells suppress experimental Sjögren syndrome by IL-33-driven expansion of ST2 + regulatory T cells
Article Snippet: Statistical analysis was done with Graphpad Prism 7. .. Recombinant human IL-2 R&D Systems Cat# 202-IL-500 Recombinant human TGF-β1 R&D Systems Cat# 240-B-010 Recombinant mouse IL-1β PeproTech Cat# 211-11B Recombinant mouse IL-4 PeproTech Cat# 214-14 Recombinant mouse IL-5 PeproTech Cat# 215-15 Recombinant mouse IL-6 PeproTech Cat# 216-16 Recombinant mouse IL-7 PeproTech Cat# 217-17 Recombinant mouse IL-12 PeproTech Cat# 210-12 Recombinant mouse IL-13 PeproTech Cat# 210-13 Recombinant mouse IL-15 PeproTech Cat# 210-15 Recombinant mouse IL-21 R&D Systems Cat# 594-ML-025 Recombinant mouse IL-22 R&D Systems Cat# 582-ML-010 Recombinant mouse IL-23 R&D Systems Cat# 1887-ML-010 Recombinant mouse/rat IL-33 R&D Systems Cat# M3300 Recombinant mouse TNF-α PeproTech Cat# 315-01A Recombinant mouse IFN-γ PeproTech Cat# 315-05 Recombinant Human IL-1β PeproTech Cat# 200-01B (5Z)-7-Oxozeaenol R&D Systems Cat# 3604/1 U0126 R&D Systems Cat# 1144/25 SB203580 R&D Systems Cat# 1202/10 SP600125 Invitrogen Cat# tlrl-sp60 JSH23 ab144824 abcam Cat#749886-87-1 PMA Sigma Aldrich Cat# P1585 Ionomycin Sigma Aldrich Cat# I0634 Golgi-Plug BD Biosciences Cat# 555029 TRIzol reagent Invitrogen Cat# 15596026 DNase I Sigma Alcrich Cat# DN25 Collagenase GIBCO Cat# 17104019 Regent or Resource source identifier RNeasy Mini Kit QIAGEN Cat# 74106 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat# 4368814 TaqMan Gene Expression Master Mix Applied Biosystems Cat# 4369016 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 Cytofix/Cytoperm Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554714 CD4+ T cell isolation kit, mouse Miltenyi Biotec Cat # 130-104-456 CD4+CD25+ Regulatory T Cell Isolation Kit, mouse Miltenyi Biotec Cat# 130-091-041 .. hADSC Human Adipose-Derived Stem Cells Catalog #: PT-5006, Lonza Walkersville Inc
Cream:Article Title: Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Recombinant Mouse GM-CSF R&D Systems Cat#415-ML Recombinant Mouse IL-4 R&D Systems Cat#404-ML Recombinant Mouse IL-23 R&D Systems Cat#1887-ML-010 Recombinant Human GM-CSF R&D Systems Cat#215-GM Recombinant Human IL-4 R&D Systems Cat#204-IL Recombinant Murine Flt3-Ligand PeproTech Cat#250-31L L-arginine Sangon Biotech Cat#A600205 L-ornithine Sigma-Aldrich Cat#O6503 L-proline Sangon Biotech Cat#A600923 Creatine Sangon Biotech Cat#A600326 L-citrulline Sangon Biotech Cat#A604057 Spermidine Sigma-Aldrich Cat#S0266 nor-NOHA Cayman Cat#1140844-63-8 Oligomycin Abcam Cat#141829 DFMO Sigma-Aldrich Cat#D193 IMQ cream MedShine Cat#120503 Halometasone cream Bright Future Pharmaceutical Laboratories, Ltd. N/A Ribonuclease A Sigma-Aldrich Cat#R6513 HNRNPA1226-237 Genescript N/A TMR-labeled HNRNPA1226-237 Genescript N/A Krt1017-28 Genescript N/A CellTrace Violet Invitrogen Cat#C34557 ionomycin Sigma-Aldrich Cat#I9657 PMA Sigma-Aldrich Cat#79346 GolgiPlug BD Biosciences Cat#555029 Critical Commercial Assays Peroxidase Substrate Kit ZSGB-BIO Cat#ZLI-9018 Protein A/G magnetic beads Merck Millipore Cat#LSKMAGAG02 CD14 Microbeads Miltenyi Biotec Cat#130-050-201 TruSeq RNA sample preparation kit Illumina Cat#FC-122-1001 TruSeq paired-end cluster kit v3-cBot-HS Illumina Cat#PE-401-3001 M-MLV First-Strand Synthesis Kit Invitrogen Cat#C28025-021 Hieff qPCR SYBR Green Master Mix Yeasen Biotech Cat#11202ES03 Minute Endosome Isolation and Cell Fractionation Kit Invent Biotech Cat#ED-028 Mouse Cytokine Array GS4000 RayBiotech Cat#GSM-CAA-4000-1 Mouse IgE ELISA MAX Deluxe BioLegend Cat#432404 Mouse IL-6 ELISA kit NeoBioscience Cat#EMC004.96 Mouse TNF-a ELISA kit NeoBioscience Cat#EMC102a.96 Mouse IFN-alpha ELISA Kit R&D Systems Cat#42120-1 Human IL-6 ELISA Kit MultiSciences Cat#70-EK106/2-96 Human TNF-a High Sensitivity ELISA Kit MultiSciences Cat#70-EK182HS-96 SimpleCHIP enzymatic chromatin immunoprecipitation kit Cell Signaling Technology Cat#9002 Duolink In Situ Red Starter Kit Mouse/Rabbit Sigma-Aldrich Cat#DUO92101-1KT OxiSelect? .. Intracellular Nitric Oxide (NO) Assay Kit Cell Biolabs Cat#STA-800 ENLITEN ATP Assay System Promega Cat#FF2000 (Continued on next page) Immunity 53, 1–13.e1–e10, July 14, 2020 e2
Magnetic Beads:Article Title: Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Recombinant Mouse GM-CSF R&D Systems Cat#415-ML Recombinant Mouse IL-4 R&D Systems Cat#404-ML Recombinant Mouse IL-23 R&D Systems Cat#1887-ML-010 Recombinant Human GM-CSF R&D Systems Cat#215-GM Recombinant Human IL-4 R&D Systems Cat#204-IL Recombinant Murine Flt3-Ligand PeproTech Cat#250-31L L-arginine Sangon Biotech Cat#A600205 L-ornithine Sigma-Aldrich Cat#O6503 L-proline Sangon Biotech Cat#A600923 Creatine Sangon Biotech Cat#A600326 L-citrulline Sangon Biotech Cat#A604057 Spermidine Sigma-Aldrich Cat#S0266 nor-NOHA Cayman Cat#1140844-63-8 Oligomycin Abcam Cat#141829 DFMO Sigma-Aldrich Cat#D193 IMQ cream MedShine Cat#120503 Halometasone cream Bright Future Pharmaceutical Laboratories, Ltd. N/A Ribonuclease A Sigma-Aldrich Cat#R6513 HNRNPA1226-237 Genescript N/A TMR-labeled HNRNPA1226-237 Genescript N/A Krt1017-28 Genescript N/A CellTrace Violet Invitrogen Cat#C34557 ionomycin Sigma-Aldrich Cat#I9657 PMA Sigma-Aldrich Cat#79346 GolgiPlug BD Biosciences Cat#555029 Critical Commercial Assays Peroxidase Substrate Kit ZSGB-BIO Cat#ZLI-9018 Protein A/G magnetic beads Merck Millipore Cat#LSKMAGAG02 CD14 Microbeads Miltenyi Biotec Cat#130-050-201 TruSeq RNA sample preparation kit Illumina Cat#FC-122-1001 TruSeq paired-end cluster kit v3-cBot-HS Illumina Cat#PE-401-3001 M-MLV First-Strand Synthesis Kit Invitrogen Cat#C28025-021 Hieff qPCR SYBR Green Master Mix Yeasen Biotech Cat#11202ES03 Minute Endosome Isolation and Cell Fractionation Kit Invent Biotech Cat#ED-028 Mouse Cytokine Array GS4000 RayBiotech Cat#GSM-CAA-4000-1 Mouse IgE ELISA MAX Deluxe BioLegend Cat#432404 Mouse IL-6 ELISA kit NeoBioscience Cat#EMC004.96 Mouse TNF-a ELISA kit NeoBioscience Cat#EMC102a.96 Mouse IFN-alpha ELISA Kit R&D Systems Cat#42120-1 Human IL-6 ELISA Kit MultiSciences Cat#70-EK106/2-96 Human TNF-a High Sensitivity ELISA Kit MultiSciences Cat#70-EK182HS-96 SimpleCHIP enzymatic chromatin immunoprecipitation kit Cell Signaling Technology Cat#9002 Duolink In Situ Red Starter Kit Mouse/Rabbit Sigma-Aldrich Cat#DUO92101-1KT OxiSelect? .. Intracellular Nitric Oxide (NO) Assay Kit Cell Biolabs Cat#STA-800 ENLITEN ATP Assay System Promega Cat#FF2000 (Continued on next page) Immunity 53, 1–13.e1–e10, July 14, 2020 e2
Sample Prep:Article Title: Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Recombinant Mouse GM-CSF R&D Systems Cat#415-ML Recombinant Mouse IL-4 R&D Systems Cat#404-ML Recombinant Mouse IL-23 R&D Systems Cat#1887-ML-010 Recombinant Human GM-CSF R&D Systems Cat#215-GM Recombinant Human IL-4 R&D Systems Cat#204-IL Recombinant Murine Flt3-Ligand PeproTech Cat#250-31L L-arginine Sangon Biotech Cat#A600205 L-ornithine Sigma-Aldrich Cat#O6503 L-proline Sangon Biotech Cat#A600923 Creatine Sangon Biotech Cat#A600326 L-citrulline Sangon Biotech Cat#A604057 Spermidine Sigma-Aldrich Cat#S0266 nor-NOHA Cayman Cat#1140844-63-8 Oligomycin Abcam Cat#141829 DFMO Sigma-Aldrich Cat#D193 IMQ cream MedShine Cat#120503 Halometasone cream Bright Future Pharmaceutical Laboratories, Ltd. N/A Ribonuclease A Sigma-Aldrich Cat#R6513 HNRNPA1226-237 Genescript N/A TMR-labeled HNRNPA1226-237 Genescript N/A Krt1017-28 Genescript N/A CellTrace Violet Invitrogen Cat#C34557 ionomycin Sigma-Aldrich Cat#I9657 PMA Sigma-Aldrich Cat#79346 GolgiPlug BD Biosciences Cat#555029 Critical Commercial Assays Peroxidase Substrate Kit ZSGB-BIO Cat#ZLI-9018 Protein A/G magnetic beads Merck Millipore Cat#LSKMAGAG02 CD14 Microbeads Miltenyi Biotec Cat#130-050-201 TruSeq RNA sample preparation kit Illumina Cat#FC-122-1001 TruSeq paired-end cluster kit v3-cBot-HS Illumina Cat#PE-401-3001 M-MLV First-Strand Synthesis Kit Invitrogen Cat#C28025-021 Hieff qPCR SYBR Green Master Mix Yeasen Biotech Cat#11202ES03 Minute Endosome Isolation and Cell Fractionation Kit Invent Biotech Cat#ED-028 Mouse Cytokine Array GS4000 RayBiotech Cat#GSM-CAA-4000-1 Mouse IgE ELISA MAX Deluxe BioLegend Cat#432404 Mouse IL-6 ELISA kit NeoBioscience Cat#EMC004.96 Mouse TNF-a ELISA kit NeoBioscience Cat#EMC102a.96 Mouse IFN-alpha ELISA Kit R&D Systems Cat#42120-1 Human IL-6 ELISA Kit MultiSciences Cat#70-EK106/2-96 Human TNF-a High Sensitivity ELISA Kit MultiSciences Cat#70-EK182HS-96 SimpleCHIP enzymatic chromatin immunoprecipitation kit Cell Signaling Technology Cat#9002 Duolink In Situ Red Starter Kit Mouse/Rabbit Sigma-Aldrich Cat#DUO92101-1KT OxiSelect? .. Intracellular Nitric Oxide (NO) Assay Kit Cell Biolabs Cat#STA-800 ENLITEN ATP Assay System Promega Cat#FF2000 (Continued on next page) Immunity 53, 1–13.e1–e10, July 14, 2020 e2
Real-time Polymerase Chain Reaction:Article Title: Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Recombinant Mouse GM-CSF R&D Systems Cat#415-ML Recombinant Mouse IL-4 R&D Systems Cat#404-ML Recombinant Mouse IL-23 R&D Systems Cat#1887-ML-010 Recombinant Human GM-CSF R&D Systems Cat#215-GM Recombinant Human IL-4 R&D Systems Cat#204-IL Recombinant Murine Flt3-Ligand PeproTech Cat#250-31L L-arginine Sangon Biotech Cat#A600205 L-ornithine Sigma-Aldrich Cat#O6503 L-proline Sangon Biotech Cat#A600923 Creatine Sangon Biotech Cat#A600326 L-citrulline Sangon Biotech Cat#A604057 Spermidine Sigma-Aldrich Cat#S0266 nor-NOHA Cayman Cat#1140844-63-8 Oligomycin Abcam Cat#141829 DFMO Sigma-Aldrich Cat#D193 IMQ cream MedShine Cat#120503 Halometasone cream Bright Future Pharmaceutical Laboratories, Ltd. N/A Ribonuclease A Sigma-Aldrich Cat#R6513 HNRNPA1226-237 Genescript N/A TMR-labeled HNRNPA1226-237 Genescript N/A Krt1017-28 Genescript N/A CellTrace Violet Invitrogen Cat#C34557 ionomycin Sigma-Aldrich Cat#I9657 PMA Sigma-Aldrich Cat#79346 GolgiPlug BD Biosciences Cat#555029 Critical Commercial Assays Peroxidase Substrate Kit ZSGB-BIO Cat#ZLI-9018 Protein A/G magnetic beads Merck Millipore Cat#LSKMAGAG02 CD14 Microbeads Miltenyi Biotec Cat#130-050-201 TruSeq RNA sample preparation kit Illumina Cat#FC-122-1001 TruSeq paired-end cluster kit v3-cBot-HS Illumina Cat#PE-401-3001 M-MLV First-Strand Synthesis Kit Invitrogen Cat#C28025-021 Hieff qPCR SYBR Green Master Mix Yeasen Biotech Cat#11202ES03 Minute Endosome Isolation and Cell Fractionation Kit Invent Biotech Cat#ED-028 Mouse Cytokine Array GS4000 RayBiotech Cat#GSM-CAA-4000-1 Mouse IgE ELISA MAX Deluxe BioLegend Cat#432404 Mouse IL-6 ELISA kit NeoBioscience Cat#EMC004.96 Mouse TNF-a ELISA kit NeoBioscience Cat#EMC102a.96 Mouse IFN-alpha ELISA Kit R&D Systems Cat#42120-1 Human IL-6 ELISA Kit MultiSciences Cat#70-EK106/2-96 Human TNF-a High Sensitivity ELISA Kit MultiSciences Cat#70-EK182HS-96 SimpleCHIP enzymatic chromatin immunoprecipitation kit Cell Signaling Technology Cat#9002 Duolink In Situ Red Starter Kit Mouse/Rabbit Sigma-Aldrich Cat#DUO92101-1KT OxiSelect? .. Intracellular Nitric Oxide (NO) Assay Kit Cell Biolabs Cat#STA-800 ENLITEN ATP Assay System Promega Cat#FF2000 (Continued on next page) Immunity 53, 1–13.e1–e10, July 14, 2020 e2
SYBR Green Assay:Article Title: Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Recombinant Mouse GM-CSF R&D Systems Cat#415-ML Recombinant Mouse IL-4 R&D Systems Cat#404-ML Recombinant Mouse IL-23 R&D Systems Cat#1887-ML-010 Recombinant Human GM-CSF R&D Systems Cat#215-GM Recombinant Human IL-4 R&D Systems Cat#204-IL Recombinant Murine Flt3-Ligand PeproTech Cat#250-31L L-arginine Sangon Biotech Cat#A600205 L-ornithine Sigma-Aldrich Cat#O6503 L-proline Sangon Biotech Cat#A600923 Creatine Sangon Biotech Cat#A600326 L-citrulline Sangon Biotech Cat#A604057 Spermidine Sigma-Aldrich Cat#S0266 nor-NOHA Cayman Cat#1140844-63-8 Oligomycin Abcam Cat#141829 DFMO Sigma-Aldrich Cat#D193 IMQ cream MedShine Cat#120503 Halometasone cream Bright Future Pharmaceutical Laboratories, Ltd. N/A Ribonuclease A Sigma-Aldrich Cat#R6513 HNRNPA1226-237 Genescript N/A TMR-labeled HNRNPA1226-237 Genescript N/A Krt1017-28 Genescript N/A CellTrace Violet Invitrogen Cat#C34557 ionomycin Sigma-Aldrich Cat#I9657 PMA Sigma-Aldrich Cat#79346 GolgiPlug BD Biosciences Cat#555029 Critical Commercial Assays Peroxidase Substrate Kit ZSGB-BIO Cat#ZLI-9018 Protein A/G magnetic beads Merck Millipore Cat#LSKMAGAG02 CD14 Microbeads Miltenyi Biotec Cat#130-050-201 TruSeq RNA sample preparation kit Illumina Cat#FC-122-1001 TruSeq paired-end cluster kit v3-cBot-HS Illumina Cat#PE-401-3001 M-MLV First-Strand Synthesis Kit Invitrogen Cat#C28025-021 Hieff qPCR SYBR Green Master Mix Yeasen Biotech Cat#11202ES03 Minute Endosome Isolation and Cell Fractionation Kit Invent Biotech Cat#ED-028 Mouse Cytokine Array GS4000 RayBiotech Cat#GSM-CAA-4000-1 Mouse IgE ELISA MAX Deluxe BioLegend Cat#432404 Mouse IL-6 ELISA kit NeoBioscience Cat#EMC004.96 Mouse TNF-a ELISA kit NeoBioscience Cat#EMC102a.96 Mouse IFN-alpha ELISA Kit R&D Systems Cat#42120-1 Human IL-6 ELISA Kit MultiSciences Cat#70-EK106/2-96 Human TNF-a High Sensitivity ELISA Kit MultiSciences Cat#70-EK182HS-96 SimpleCHIP enzymatic chromatin immunoprecipitation kit Cell Signaling Technology Cat#9002 Duolink In Situ Red Starter Kit Mouse/Rabbit Sigma-Aldrich Cat#DUO92101-1KT OxiSelect? .. Intracellular Nitric Oxide (NO) Assay Kit Cell Biolabs Cat#STA-800 ENLITEN ATP Assay System Promega Cat#FF2000 (Continued on next page) Immunity 53, 1–13.e1–e10, July 14, 2020 e2
Isolation:Article Title: Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Recombinant Mouse GM-CSF R&D Systems Cat#415-ML Recombinant Mouse IL-4 R&D Systems Cat#404-ML Recombinant Mouse IL-23 R&D Systems Cat#1887-ML-010 Recombinant Human GM-CSF R&D Systems Cat#215-GM Recombinant Human IL-4 R&D Systems Cat#204-IL Recombinant Murine Flt3-Ligand PeproTech Cat#250-31L L-arginine Sangon Biotech Cat#A600205 L-ornithine Sigma-Aldrich Cat#O6503 L-proline Sangon Biotech Cat#A600923 Creatine Sangon Biotech Cat#A600326 L-citrulline Sangon Biotech Cat#A604057 Spermidine Sigma-Aldrich Cat#S0266 nor-NOHA Cayman Cat#1140844-63-8 Oligomycin Abcam Cat#141829 DFMO Sigma-Aldrich Cat#D193 IMQ cream MedShine Cat#120503 Halometasone cream Bright Future Pharmaceutical Laboratories, Ltd. N/A Ribonuclease A Sigma-Aldrich Cat#R6513 HNRNPA1226-237 Genescript N/A TMR-labeled HNRNPA1226-237 Genescript N/A Krt1017-28 Genescript N/A CellTrace Violet Invitrogen Cat#C34557 ionomycin Sigma-Aldrich Cat#I9657 PMA Sigma-Aldrich Cat#79346 GolgiPlug BD Biosciences Cat#555029 Critical Commercial Assays Peroxidase Substrate Kit ZSGB-BIO Cat#ZLI-9018 Protein A/G magnetic beads Merck Millipore Cat#LSKMAGAG02 CD14 Microbeads Miltenyi Biotec Cat#130-050-201 TruSeq RNA sample preparation kit Illumina Cat#FC-122-1001 TruSeq paired-end cluster kit v3-cBot-HS Illumina Cat#PE-401-3001 M-MLV First-Strand Synthesis Kit Invitrogen Cat#C28025-021 Hieff qPCR SYBR Green Master Mix Yeasen Biotech Cat#11202ES03 Minute Endosome Isolation and Cell Fractionation Kit Invent Biotech Cat#ED-028 Mouse Cytokine Array GS4000 RayBiotech Cat#GSM-CAA-4000-1 Mouse IgE ELISA MAX Deluxe BioLegend Cat#432404 Mouse IL-6 ELISA kit NeoBioscience Cat#EMC004.96 Mouse TNF-a ELISA kit NeoBioscience Cat#EMC102a.96 Mouse IFN-alpha ELISA Kit R&D Systems Cat#42120-1 Human IL-6 ELISA Kit MultiSciences Cat#70-EK106/2-96 Human TNF-a High Sensitivity ELISA Kit MultiSciences Cat#70-EK182HS-96 SimpleCHIP enzymatic chromatin immunoprecipitation kit Cell Signaling Technology Cat#9002 Duolink In Situ Red Starter Kit Mouse/Rabbit Sigma-Aldrich Cat#DUO92101-1KT OxiSelect? .. Intracellular Nitric Oxide (NO) Assay Kit Cell Biolabs Cat#STA-800 ENLITEN ATP Assay System Promega Cat#FF2000 (Continued on next page) Immunity 53, 1–13.e1–e10, July 14, 2020 e2
Cell Fractionation:Article Title: Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Recombinant Mouse GM-CSF R&D Systems Cat#415-ML Recombinant Mouse IL-4 R&D Systems Cat#404-ML Recombinant Mouse IL-23 R&D Systems Cat#1887-ML-010 Recombinant Human GM-CSF R&D Systems Cat#215-GM Recombinant Human IL-4 R&D Systems Cat#204-IL Recombinant Murine Flt3-Ligand PeproTech Cat#250-31L L-arginine Sangon Biotech Cat#A600205 L-ornithine Sigma-Aldrich Cat#O6503 L-proline Sangon Biotech Cat#A600923 Creatine Sangon Biotech Cat#A600326 L-citrulline Sangon Biotech Cat#A604057 Spermidine Sigma-Aldrich Cat#S0266 nor-NOHA Cayman Cat#1140844-63-8 Oligomycin Abcam Cat#141829 DFMO Sigma-Aldrich Cat#D193 IMQ cream MedShine Cat#120503 Halometasone cream Bright Future Pharmaceutical Laboratories, Ltd. N/A Ribonuclease A Sigma-Aldrich Cat#R6513 HNRNPA1226-237 Genescript N/A TMR-labeled HNRNPA1226-237 Genescript N/A Krt1017-28 Genescript N/A CellTrace Violet Invitrogen Cat#C34557 ionomycin Sigma-Aldrich Cat#I9657 PMA Sigma-Aldrich Cat#79346 GolgiPlug BD Biosciences Cat#555029 Critical Commercial Assays Peroxidase Substrate Kit ZSGB-BIO Cat#ZLI-9018 Protein A/G magnetic beads Merck Millipore Cat#LSKMAGAG02 CD14 Microbeads Miltenyi Biotec Cat#130-050-201 TruSeq RNA sample preparation kit Illumina Cat#FC-122-1001 TruSeq paired-end cluster kit v3-cBot-HS Illumina Cat#PE-401-3001 M-MLV First-Strand Synthesis Kit Invitrogen Cat#C28025-021 Hieff qPCR SYBR Green Master Mix Yeasen Biotech Cat#11202ES03 Minute Endosome Isolation and Cell Fractionation Kit Invent Biotech Cat#ED-028 Mouse Cytokine Array GS4000 RayBiotech Cat#GSM-CAA-4000-1 Mouse IgE ELISA MAX Deluxe BioLegend Cat#432404 Mouse IL-6 ELISA kit NeoBioscience Cat#EMC004.96 Mouse TNF-a ELISA kit NeoBioscience Cat#EMC102a.96 Mouse IFN-alpha ELISA Kit R&D Systems Cat#42120-1 Human IL-6 ELISA Kit MultiSciences Cat#70-EK106/2-96 Human TNF-a High Sensitivity ELISA Kit MultiSciences Cat#70-EK182HS-96 SimpleCHIP enzymatic chromatin immunoprecipitation kit Cell Signaling Technology Cat#9002 Duolink In Situ Red Starter Kit Mouse/Rabbit Sigma-Aldrich Cat#DUO92101-1KT OxiSelect? .. Intracellular Nitric Oxide (NO) Assay Kit Cell Biolabs Cat#STA-800 ENLITEN ATP Assay System Promega Cat#FF2000 (Continued on next page) Immunity 53, 1–13.e1–e10, July 14, 2020 e2
Enzyme-linked Immunosorbent Assay:Article Title: Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Recombinant Mouse GM-CSF R&D Systems Cat#415-ML Recombinant Mouse IL-4 R&D Systems Cat#404-ML Recombinant Mouse IL-23 R&D Systems Cat#1887-ML-010 Recombinant Human GM-CSF R&D Systems Cat#215-GM Recombinant Human IL-4 R&D Systems Cat#204-IL Recombinant Murine Flt3-Ligand PeproTech Cat#250-31L L-arginine Sangon Biotech Cat#A600205 L-ornithine Sigma-Aldrich Cat#O6503 L-proline Sangon Biotech Cat#A600923 Creatine Sangon Biotech Cat#A600326 L-citrulline Sangon Biotech Cat#A604057 Spermidine Sigma-Aldrich Cat#S0266 nor-NOHA Cayman Cat#1140844-63-8 Oligomycin Abcam Cat#141829 DFMO Sigma-Aldrich Cat#D193 IMQ cream MedShine Cat#120503 Halometasone cream Bright Future Pharmaceutical Laboratories, Ltd. N/A Ribonuclease A Sigma-Aldrich Cat#R6513 HNRNPA1226-237 Genescript N/A TMR-labeled HNRNPA1226-237 Genescript N/A Krt1017-28 Genescript N/A CellTrace Violet Invitrogen Cat#C34557 ionomycin Sigma-Aldrich Cat#I9657 PMA Sigma-Aldrich Cat#79346 GolgiPlug BD Biosciences Cat#555029 Critical Commercial Assays Peroxidase Substrate Kit ZSGB-BIO Cat#ZLI-9018 Protein A/G magnetic beads Merck Millipore Cat#LSKMAGAG02 CD14 Microbeads Miltenyi Biotec Cat#130-050-201 TruSeq RNA sample preparation kit Illumina Cat#FC-122-1001 TruSeq paired-end cluster kit v3-cBot-HS Illumina Cat#PE-401-3001 M-MLV First-Strand Synthesis Kit Invitrogen Cat#C28025-021 Hieff qPCR SYBR Green Master Mix Yeasen Biotech Cat#11202ES03 Minute Endosome Isolation and Cell Fractionation Kit Invent Biotech Cat#ED-028 Mouse Cytokine Array GS4000 RayBiotech Cat#GSM-CAA-4000-1 Mouse IgE ELISA MAX Deluxe BioLegend Cat#432404 Mouse IL-6 ELISA kit NeoBioscience Cat#EMC004.96 Mouse TNF-a ELISA kit NeoBioscience Cat#EMC102a.96 Mouse IFN-alpha ELISA Kit R&D Systems Cat#42120-1 Human IL-6 ELISA Kit MultiSciences Cat#70-EK106/2-96 Human TNF-a High Sensitivity ELISA Kit MultiSciences Cat#70-EK182HS-96 SimpleCHIP enzymatic chromatin immunoprecipitation kit Cell Signaling Technology Cat#9002 Duolink In Situ Red Starter Kit Mouse/Rabbit Sigma-Aldrich Cat#DUO92101-1KT OxiSelect? .. Intracellular Nitric Oxide (NO) Assay Kit Cell Biolabs Cat#STA-800 ENLITEN ATP Assay System Promega Cat#FF2000 (Continued on next page) Immunity 53, 1–13.e1–e10, July 14, 2020 e2
In Situ:Article Title: Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Recombinant Mouse GM-CSF R&D Systems Cat#415-ML Recombinant Mouse IL-4 R&D Systems Cat#404-ML Recombinant Mouse IL-23 R&D Systems Cat#1887-ML-010 Recombinant Human GM-CSF R&D Systems Cat#215-GM Recombinant Human IL-4 R&D Systems Cat#204-IL Recombinant Murine Flt3-Ligand PeproTech Cat#250-31L L-arginine Sangon Biotech Cat#A600205 L-ornithine Sigma-Aldrich Cat#O6503 L-proline Sangon Biotech Cat#A600923 Creatine Sangon Biotech Cat#A600326 L-citrulline Sangon Biotech Cat#A604057 Spermidine Sigma-Aldrich Cat#S0266 nor-NOHA Cayman Cat#1140844-63-8 Oligomycin Abcam Cat#141829 DFMO Sigma-Aldrich Cat#D193 IMQ cream MedShine Cat#120503 Halometasone cream Bright Future Pharmaceutical Laboratories, Ltd. N/A Ribonuclease A Sigma-Aldrich Cat#R6513 HNRNPA1226-237 Genescript N/A TMR-labeled HNRNPA1226-237 Genescript N/A Krt1017-28 Genescript N/A CellTrace Violet Invitrogen Cat#C34557 ionomycin Sigma-Aldrich Cat#I9657 PMA Sigma-Aldrich Cat#79346 GolgiPlug BD Biosciences Cat#555029 Critical Commercial Assays Peroxidase Substrate Kit ZSGB-BIO Cat#ZLI-9018 Protein A/G magnetic beads Merck Millipore Cat#LSKMAGAG02 CD14 Microbeads Miltenyi Biotec Cat#130-050-201 TruSeq RNA sample preparation kit Illumina Cat#FC-122-1001 TruSeq paired-end cluster kit v3-cBot-HS Illumina Cat#PE-401-3001 M-MLV First-Strand Synthesis Kit Invitrogen Cat#C28025-021 Hieff qPCR SYBR Green Master Mix Yeasen Biotech Cat#11202ES03 Minute Endosome Isolation and Cell Fractionation Kit Invent Biotech Cat#ED-028 Mouse Cytokine Array GS4000 RayBiotech Cat#GSM-CAA-4000-1 Mouse IgE ELISA MAX Deluxe BioLegend Cat#432404 Mouse IL-6 ELISA kit NeoBioscience Cat#EMC004.96 Mouse TNF-a ELISA kit NeoBioscience Cat#EMC102a.96 Mouse IFN-alpha ELISA Kit R&D Systems Cat#42120-1 Human IL-6 ELISA Kit MultiSciences Cat#70-EK106/2-96 Human TNF-a High Sensitivity ELISA Kit MultiSciences Cat#70-EK182HS-96 SimpleCHIP enzymatic chromatin immunoprecipitation kit Cell Signaling Technology Cat#9002 Duolink In Situ Red Starter Kit Mouse/Rabbit Sigma-Aldrich Cat#DUO92101-1KT OxiSelect? .. Intracellular Nitric Oxide (NO) Assay Kit Cell Biolabs Cat#STA-800 ENLITEN ATP Assay System Promega Cat#FF2000 (Continued on next page) Immunity 53, 1–13.e1–e10, July 14, 2020 e2
Cell Stimulation:
Transfection:
Protease Inhibitor:
Immunohistochemistry:
cDNA Synthesis:
Polymerase Chain Reaction:
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